Effect of depletion of TRIP12 on gene expression in adult murine pancreatic acinar cells and murine pancreatic cancer cell lines. Effect of depletion of TRIP12 on gene expression in adult murine pancreatic acinar cells and murine pancreatic cancer cell lines

To investigate the role of the E3 ubiquitin ligase Thyroid Receptor Interacting Protein 12 (TRIP12) in pancreatic acinar cell identity and pancreatic carcinogenesis, we used genetically engineered mouse models of pancreas-selective Trip12 deletion, mutant Kras (G12D) and mutant P53 (R172H). We performed gene expression analysis using RNA-seq data from adult acinar cells. We established cell lines from murine pancreatic tumors. Overall design: Comparative gene expression analysis of RNA-seq data from three TRIP12 knockout and three TRIP12 expressing pancreatic acinar cells. Comparative gene expression analysis of RNA-Seq data from three cell lines established from two Trip12 expressing KPC pancreatic murine tumors, from two Trip12 knockout KPC tumors and from two Trip12 heterozygous KPC tumors.