Phenylpropanoid Carbon Allocation and MYB/NAC Regulatory Remodeling Underlie Stem Lignification and Structural Reinforcement in Impatiens Species
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Oligonucleotide primers used for quantitative real-time PCR (qRT-PCR) validation (Table S1); Summary of RNA-sequencing quality and read-mapping statistics for individual samples (Table S2); Differentially expressed genes between I. balsamina and I. clavigera groups, with expression profiles and functional annotations (Table S3); Differentially accumulated metabolites between I. balsamina and I. clavigera groups, with abundance profiles and statistical parameters (Table S4); Candidate genes associated with lignin biosynthesis in the I. balsamina versus I. clavigera comparison (Table S5); Pearson correlations between lignin-biosynthesis-related differentially expressed genes and lignin-pathway metabolites (Table S6); Candidate MYB and NAC transcription factor genes associated with lignin biosynthesis (Table S7); Pearson correlations between lignin-biosynthesis-related genes and MYB or NAC transcription factor genes (Table S8) ; Transcriptomic KEGG and GO analyses between I. balsamina and I. clavigera. (Figure S1); Metabolomic KEGG analysis between I. balsamina and I. clavigera (Figure S2); Evolutionary and structural features of MYB and NAC transcription factor families in Impatiens (Figure S3); Phylogenetic relationships of MYB and NAC transcription factor families between Impatiens and Arabidopsis (Figure S4); Correlation of log2FC values derived from qRT-PCR and RNA-seq across contrasts (Figure S5)
用于实时定量PCR（quantitative real-time PCR, qRT-PCR）验证的寡核苷酸引物（见表S1）；各样品RNA测序质量评估及reads比对统计汇总（见表S2）；凤仙花（I. balsamina）与棒凤仙花（I. clavigera）组间的差异表达基因，附带表达谱与功能注释信息（见表S3）；凤仙花与棒凤仙花组间的差异积累代谢物，附带丰度谱与统计参数（见表S4）；凤仙花与棒凤仙花比较组中与木质素生物合成相关的候选基因（见表S5）；木质素生物合成相关差异表达基因与木质素通路代谢物间的皮尔逊相关系数（见表S6）；与木质素生物合成相关的候选MYB及NAC转录因子基因（见表S7）；木质素生物合成相关基因与MYB或NAC转录因子基因间的皮尔逊相关系数（见表S8）；凤仙花与棒凤仙花间的转录组KEGG（Kyoto Encyclopedia of Genes and Genomes，京都基因与基因组百科全书）及GO（Gene Ontology，基因本体论）富集分析（图S1）；凤仙花与棒凤仙花间的代谢组KEGG富集分析（图S2）；凤仙花属MYB及NAC转录因子家族的进化与结构特征（图S3）；凤仙花属与拟南芥（Arabidopsis）间MYB及NAC转录因子家族的系统发育关系（图S4）；各比较组中实时定量PCR与RNA测序所得log2FC值的相关性分析（图S5）
