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E. coli sequencing after various editing procedures

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NIAID Data Ecosystem2026-05-01 收录
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Sequencing of a large number of strains of E. coli after various editing procedures revealed that CRISPR/Cas9-assisted lambda Red recombineering elevated the percentage of colonies containing unintended mutations from a background of ~7-10% to 26%. These unintended mutations could have phenotypic effects that mask the effects of intended edits.

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2024-03-14
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