Expression analysis of aging cardiac tubes in Drosophila

Investigation of the whole genome expression levels from dissected adult cardiac tubes at 10 days and 40 day. We describe a functional genomic investigation of the genetic control of cardiac senescence in Drosophila. Molecular signatures of heart aging were identified by differential transcriptome analysis followed by a detailed bio-informatic analysis. This approach implicated the JNK/dJun pathway and the transcription factor Vri/dNFIL3 in the transcription regulatory network involved in cardiac senescence and suggested the possible involvement of oxidative stress (OS) in the aging process. 4 biological replicates for each time point (10 days and 40 days) were used. Dye-swap replications, in which each hybridization is done twice, with dye assignments reversed in the second hybridization, were used with a design which allows direct comparison between time points . This method allows technical replicates and eliminates variation that might result from differences in fluorescence dye intensities.