The Zinc Finger Transcription Factor Sp9 Is Required for the Development of Striatopallidal Projection Neurons

Purpose: To assess changes in gene expression profiles from the P0 littermate Sp9Flox/Flox control striatum (including SVZ) and conditional mutant striatum of Drd2-Cre; Sp9Flox/Flox mice. Methods: total RNA was isolated and sequenced from the striatum of the P0 littermate Sp9Flox/Flox control or conditional mutant striatum of Drd2-Cre; Sp9Flox/Flox mice in triplicate using an Illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered changed which demonstrated fold-change>=2, and p value <=0.05. Changed genes were then filtered to reveal Sp9 downstream targets which were altered in vivo due to the loss of Sp9 expression. Results: 179 genes were significantly increased and 147 genes were significantly decreased in expression level due to the loss of Sp9 expression. total RNA was isolated and sequenced from the striatum of the P0 littermate Sp9Flox/Flox control or conditional mutant striatum of Drd2-Cre; Sp9Flox/Flox mice in triplicate using an Illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered changed which demonstrated fold-change>=2, and p value <=0.05. Changed genes were then filtered to reveal Sp9 downstream targets which were altered in vivo due to the loss of Sp9 expression.目的：评估P0胎龄同窝Sp9Flox/Flox对照纹状体（包含室管膜下区（Subventricular Zone，SVZ））与Drd2-Cre; Sp9Flox/Flox条件性突变小鼠纹状体的基因表达谱变化。 方法：提取P0胎龄同窝Sp9Flox/Flox对照小鼠及Drd2-Cre; Sp9Flox/Flox条件性突变小鼠纹状体的总RNA，每组设置3次生物学重复，采用Illumina HiSeq 2500平台进行高通量测序；原始测序数据通过TopHat进行分析。以倍数变化（fold-change）≥2且p值≤0.05作为基因表达显著改变的判定标准；随后对差异表达基因进行筛选，以获取因Sp9表达缺失而在体内发生表达改变的Sp9下游靶基因。 结果：因Sp9表达缺失，纹状体中共有179个基因表达显著上调，147个基因表达显著下调。