Bacillus coagulans GBI-30, 6086 spores modulates intestinal microbiota and changes health parameters in rats

The aim of this study was to investigate the impact of the type different of probiotic food matrixes (orange juice and yogurt) on the persistence/functionality of spore-forming probiotic microorganism (Bacillus coagulans GBI-30, 6086) on microbial ecology and health parameters of male rats. For this purpose, 48 male rats were divided into 6 groups: control group received distilled water; juice group received orange juice; yogurt group received yogurt; probiotic Bacillus group received Bacillus probiotic suspended in distilled water; juice probiotic group received probiotic orange juice and yogurt probiotic group received probiotic yogurt. All animals received 4 mL/day of each food matrix by gavage during twenty one days. Then, the animals were sacrificed and the gastrocnemius muscle, cecal content and blood were collected and analyzed.