Stepwise Requirements for Polymerases Theta and Delta in TMEJ (NCS Treated samples)

Coordinate data for STORM performed in association with DOI: https://doi.org/10.21203/rs.3.rs-2057977. NCS-treated RPE1 cells expressing a HALO-tagged POLQ. 53BP1 was imaged in green, HALO-POLQ in red, EdU in dark red, and POLD1 in blue.本数据集对应关联DOI：https://doi.org/10.21203/rs.3.rs-2057977的随机光学重建显微镜（STORM，Stochastic Optical Reconstruction Microscopy）成像实验，包含其成像坐标数据。实验样本为经NCS处理、表达带有HALO标签的POLQ（DNA聚合酶θ）的RPE1细胞（视网膜色素上皮细胞系1，Retinal Pigment Epithelial cell line 1）。成像荧光标记方案如下：53BP1（p53结合蛋白1，p53-binding protein 1）以绿色荧光成像，HALO-POLQ以红色荧光成像，EdU（5-乙炔基-2'-脱氧尿苷）以暗红色荧光成像，POLD1（DNA聚合酶δ催化亚基1）以蓝色荧光成像。