Genotyping by sequencing of tomato

The US Department of Agriculture, Agricultural Research Service, Plant Genetic Resources Unit (PGRU) conserves 6,610 tomato accessions in the form of publicly available seed stocks. These are distributed throughout the world to requesters for purposes of breeding, research, and higher education. We performed genotyping by sequencing on 190 tomato accessions from PGRUs collection. These originated from 31 countries and included various usage types such as fresh market, ornamental, processing, breeders lines, landraces, and home gardening types. The results of this study provided gene discovery tools and other genetic information that can be used to increase the efficiency of choosing genetic stocks for tomato research and breeding.