Complex I composition and C. elegans homology.

82% (31/38) of mammalian, nuclear DNA-encoded, complex I subunits demonstrate extensive homology across evolution, ranging from 41.5% to 99.2% similarity between C. elegans and human proteins. Mitochondrial DNA-encoded complex I subunits, although highly conserved, were not included in this study.*indicates human subunits not conserved in C. elegans.**indicates subunits in which pathogenic mutations have been identified in human patients.#gas-1(fc21) missense mutant allelic to K09A9.5 RNAi knockdown.&two RNAi clones identified in silico as NDUFA2 homologues.asubcomplexes are defined per centrifugation fractions in bovine mitochondria, which generally correspond to structural sublocalization: I��, matrix arm (I��) plus several additional membrane bound subunits; I��, membrane-bound arm; I��, matrix arm only; I��, undefined location identified in ��breakthrough centrifugation fraction�� (4, 14).