Archaeal Connectase is a specific and efficient protein ligase related to proteasome β subunits

Primary data used in the study on Connectase, a novel protein ligase enzyme. The paper can be found under https://www.biorxiv.org/content/10.1101/2020.08.14.217505v1 and is soon to be published by PNAS (will be linked here); the main text and the method section give detailed information on how the data was generated and how it can be interpreted. Supplied here are full images of all polyacrylamide gels, their quantitative analysis and data recorded in mass spectrometry, light scattering and microscale thermophoresis analyses. All files are named to indicate the experiment and/or figure, in which they were used. The polyacrylamide gels are supplied as TIF files and all other data as XLSX tables. The quantification tables include calculated ligation rates as they are plotted in the paper. The tables for the mass spectrometric analyses of the dimethyl labeling and pulldown experiments show all detected peptides, their intensities and further information as indicated by the headers. The tables for the light scattering, liquid chromatography mass spectrometry (LCMS) and microscale thermophoresis experiments contain x/y data used for plots in the paper. In the LCMS tables, x denotes the deconvoluted molecular mass [Da] and y the signal intensity [AU]. In the light scattering tables, x denotes the elution volume [ml] from the column and y the signal intensity [AU] or detected molar mass [DA] as indicated. In the microscale thermophoresis tables, x denotes the substrate concentration [nM] and y the detected signal in three independent experiments or a data fit as approximated by the instrument software.本数据集用于一项针对新型蛋白质连接酶Connectase的研究。相关论文可通过链接https://www.biorxiv.org/content/10.1101/2020.08.14.217505v1获取，该论文即将由PNAS（美国国家科学院院刊）刊发，后续将在此处补充其正式链接。研究的正文与方法部分详细说明了数据集的生成方式与解读方法。 本次提供的数据集包含所有聚丙烯酰胺凝胶（polyacrylamide gels）的完整图像、凝胶定量分析结果，以及质谱（mass spectrometry）、光散射（light scattering）和微量热泳动（microscale thermophoresis）分析得到的实验数据。所有文件均以其所对应实验或图表的名称进行命名。 聚丙烯酰胺凝胶图像以TIF格式文件提供，其余数据均以XLSX表格形式存储。定量分析表格包含了论文绘图所用的计算得到的连接速率数据。针对二甲基标记与下拉实验（pulldown experiments）的质谱分析表格，列出了所有检测到的肽段（peptides）、其信号强度，以及表头标注的其他相关信息。 光散射、液相色谱-质谱联用（liquid chromatography mass spectrometry, LCMS）以及微量热泳动实验的表格，包含了论文绘图所用的x/y轴数据。在LCMS表格中，x轴代表解卷积后的分子质量[Da]，y轴代表信号强度[AU]。在光散射表格中，x轴代表色谱柱洗脱体积[ml]，y轴代表信号强度[AU]或检测到的摩尔质量[Da]，具体以表格标注为准。在微量热泳动表格中，x轴代表底物浓度[nM]，y轴代表三次独立重复实验的检测信号，或由仪器软件拟合得到的数据结果。