Effect of cardiac specific depletion of one allele of Mrps5 on gene expression in heart at postnatal day 4

To investigate the function of reduction in Mrps5 expression in the regulation of cardiomyocyte proliferation and postnatal heart development, we established the mouse with cardiac specific depletion of one allele of Mrps5 through Cre-Loxp system by crossing Mrps5 fl/fl mouse with Myh6-Cre mouse. We cross Mrps5 fl/fl mouse with Myh6-Cre mouse to obtain the Mrps5 fl/+;Myh6-Cre mouse and its control littermates Mrps5 fl/+ mouse. So that one allele of Mrps5 gene was depleted upon Myh6 gene promoter drivened Cre expression at the later stage of embyronic stage. We then performed gene expression profiling analysis using data obtained from RNA-seq of Mrps5 fl/+; Myh6-Cre and Mrps5 fl/+ mouse hearts at postnatal day 4. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Mrps5 fl/+; Myh6-Cre mouse hearts and its control littermates hearts at postnatal day 4. Each group has 4 biological replicates, the control group indicates as Ctrl_1, Ctrl_2, Ctrl_3, Ctrl_4 and the Mrps5 heterozygous group indicates as cHET_1, cHET_2, cHET_3, cHET_4.