KOSMOS 2018 Gran Canaria mesocosm study on artificial upwelling: pigment concentration and pigment-based phytoplankton community composition@en

Pigment concentration and pigment-based phytoplankton community composition data from a KOSMOS mesocosm experiment carried out in the frame work of the Ocean Artificial Upwelling project. The experiment was performed in the North-East Atlantic Ocean off the coast of Gran Canaria in autumn 2018 and lasted for 39 days. In this study we investigated the effect of different intensities of artificial upwelling combined with two upwelling modes (recurring additions versus one singular addition) on the phytoplankton community. Filters were analysed for a range of photosynthetic pigments using reverse-phase high-performance liquid chromatography (HPLC). Based on pigment concentrations, phytoplankton community composition was approximated using the CHEMTAX software with the original pigment ratios from Mackey et al (1996, doi:10.3354/meps144265). The input included Chl a, b, c2, and c3, peridinin, 19'-butanoyloxyfucoxanthin, fucoxanthin, neoxanthin, prasinoxanthin, violaxanthin, 19'-hexanoyloxyfucoxanthin, alloxanthin, diadinoxanthin, diatoxanthin and zeaxanthin. Divinyl Chl a was instead fully associated with Prochlorophyceae. The presence of the main phytoplankton groups is expressed in Chl a equivalents.本数据集包含“海洋人工上升流（Ocean Artificial Upwelling）”项目框架下开展的KOSMOS中型实验生态系统（mesocosm）实验所产生的色素浓度与基于色素的浮游植物群落组成数据。该实验于2018年秋季在大西洋东北部大加那利岛沿岸海域实施，持续时长共计39天。本研究旨在探究不同强度的人工上升流，配合两种上升流模式（周期性补充与单次补充）对浮游植物群落的影响。研究人员采用反相高效液相色谱（reverse-phase high-performance liquid chromatography, HPLC）对滤膜样本中的多种光合色素进行分析。基于色素浓度数据，借助CHEMTAX软件，结合Mackey等人1996年发表的原始色素比值（doi:10.3354/meps144265），对浮游植物群落组成进行估算。本次分析的输入色素包括叶绿素a（Chl a）、叶绿素b（Chl b）、叶绿素c2（Chl c2）、叶绿素c3（Chl c3）、多甲藻素（peridinin）、19'-丁酰氧岩藻黄素（19'-butanoyloxyfucoxanthin）、岩藻黄素（fucoxanthin）、新黄素（neoxanthin）、普拉斯诺黄素（prasinoxanthin）、紫黄素（violaxanthin）、19'-己酰氧岩藻黄素（19'-hexanoyloxyfucoxanthin）、别藻黄素（alloxanthin）、双甲藻黄素（diadinoxanthin）、双甲藻黄质（diatoxanthin）与玉米黄质（zeaxanthin）。二乙烯基叶绿素a（Divinyl Chl a）则完全归属于原绿藻门（Prochlorophyceae）。主要浮游植物类群的相对丰度以叶绿素a当量（Chl a equivalents）形式表征。