Glue Grant 2 Transcriptomic Cohort of Critically Injured Trauma Patients

The Glue Grant 2 transcriptomic cohort is a multicenter prospective observational cohort of critically injured trauma patients enrolled from seven tertiary care trauma centers in the United States. The cohort includes 243 adult trauma patients with severe injury, defined by an Injury Severity Score (ISS) ≥16 and requirement for intensive care unit admission, together with 57 age- and sex-matched healthy volunteers as controls. Participating centers included the University of Florida, University of Pittsburgh Medical Center, University of Washington, University of California San Francisco, The University of Texas Southwestern Medical Center, Massachusetts General Hospital, and the University of Colorado Anschutz Medical Campus. Peripheral blood samples were collected longitudinally at predefined post-injury time points, including early injury, intermediate recovery phases, and recovery or hospital discharge when available. Transcriptomic profiling was performed on circulating immune-cell populations and linked to prospectively collected clinical metadata, including ICU length of stay, organ failure, survival status, and recovery-related outcomes. In this study, the Glue Grant 2 cohort was used to develop and evaluate immune recovery clock models and to investigate the relationship between post-trauma immune transcriptional dynamics and clinical outcomes.Glue Grant 2转录组队列是一项多中心前瞻性观察队列，纳入了来自美国7家三级创伤中心的重症创伤患者。该队列包含243例重度创伤成年患者（重度创伤定义为损伤严重程度评分（Injury Severity Score, ISS）≥16且需入住重症监护病房（Intensive Care Unit, ICU）），以及57例年龄与性别匹配的健康志愿者作为对照。参与本研究的中心包括佛罗里达大学、匹兹堡大学医学中心、华盛顿大学、加州大学旧金山分校、德克萨斯大学西南医学中心、麻省总医院以及科罗拉多大学安舒茨医学校区。 研究人员在预设的伤后时间点纵向采集外周血样本，涵盖损伤早期、恢复中期，以及可获取的恢复阶段或出院时间点。研究对循环免疫细胞群开展了转录组谱分析（transcriptomic profiling），并将其与前瞻性收集的临床元数据相关联，这些元数据包括ICU住院时长、器官功能衰竭情况、生存状态以及恢复相关结局。 本研究使用Glue Grant 2队列开发并评估免疫恢复时钟模型，同时探究创伤后免疫转录动态与临床结局之间的关联。