Data for: Selection and Validation of Reference Genes for Quantitative real-time PCR in Rosmarinus officinalis in various tissues and under elicitation

Fig. S1. Melt curves associated with each of reference gene; gene-specific amplifications is confirmed by the presence of a single peak. (A) Melt curve for 18S rRNA and α-TUB genes; (B) Melt curve for β-TUB and EF1-a genes; (C) Melt curve for GAPDH and CYP genes; (D) Melt curve for ACT gene. The melt curves of each gene are related to three technical replicates. Fig. S2. The specificity of primer pairs for Q-PCR amplification. Agarose gel (1.5%) electrophoresis showing PCR amplified products of the expected size for seven candidate reference genes. M: 1kb marker.补充图S1：各内参基因（reference gene）的熔解曲线（melt curve）；单一熔峰的出现可证实基因特异性扩增。(A) 18S rRNA与α-TUB基因的熔解曲线；(B) β-TUB与EF1-α基因的熔解曲线；(C) GAPDH与CYP基因的熔解曲线；(D) ACT基因的熔解曲线。各基因的熔解曲线均对应3次技术重复（technical replicates）。补充图S2：定量PCR（Q-PCR）扩增引物对的特异性。1.5%琼脂糖凝胶电泳（agarose gel electrophoresis）结果显示，7个候选内参基因的PCR扩增产物均符合预期分子量大小。M：1kb分子量标记物（1kb marker）。