Shedding and degradation rates of environmental DNA (eDNA) from crown-of-thorns seastar (AIMS)

This dataset consists of shedding and degradation rates of environmental DNA (eDNA) from crown-of-thorns seastar (CoTS) under different feeding and temperature treatments, based on aquarium and laboratory experiments.The aim of this study was to improve the understanding of the major factors affecting the dynamics of environmental DNA (eDNA) from crown-of-thorns seastar (CoTS). This data will help inform the use of eDNA as a novel tool for the management of CoTS outbreaks on tropical coral reefs. This dataset consists of two spread sheets. The description of each is provided below.‘shedding’, cots_edna.csvThis spread sheet consists of the eDNA shedding rates (DNA copies per hour per individual) of 8-month-old juvenile CoTS under two temperatures (24 and 28 oC) and three feeding treatments(no coral, coral accessible and coral inaccessible). ‘degradation’, cots_edna.csvThis spread sheet consists of the eDNA concentration (DNA copies per litre) at designated time points between 0 – 120 hours, under three temperatures (24, 26 and 28 oC). -Temp: water temperature in oC-Treatment: feeding treatment (NF = no food, FA = food accessible, FI = food inaccessible)-Flow: flow rate of the tank (litre per minute)-Diameter: diameter of the CoTS in mm-Weight: weight of the CoTS in mg-SheddingRate: in the unit of DNA copies per hour

本数据集基于水族箱与实验室实验，涵盖了长棘海星（crown-of-thorns seastar, CoTS）的环境DNA（environmental DNA, eDNA）脱落速率与降解速率，实验设置了不同的投喂与温度处理条件。
本研究旨在加深对影响长棘海星环境DNA动态变化的关键因素的认知。本数据集将为将环境DNA作为新型工具，用于管控热带珊瑚礁上的长棘海星暴发灾害提供数据支撑。
本数据集包含两份电子数据表，具体说明如下：
“脱落速率”工作表（cots_edna.csv）：该表记录了8月龄幼体长棘海星在2种温度（24与28摄氏度）与3种投喂处理（无珊瑚投喂、可接触珊瑚投喂、不可接触珊瑚投喂）下的eDNA脱落速率，单位为每小时每个体的DNA拷贝数。
“降解速率”工作表（cots_edna.csv）：该表记录了在3种温度（24、26与28摄氏度）条件下，0至120小时内各指定时间点的eDNA浓度，单位为每升DNA拷贝数。
各字段的详细说明如下：
- Temp：水温，单位为摄氏度（℃）
- Treatment：投喂处理类型（NF=无食物，FA=可获取食物，FI=不可获取食物）
- Flow：养殖水箱的水流速率，单位为升每分钟
- Diameter：长棘海星的体径，单位为毫米（mm）
- Weight：长棘海星的体重，单位为毫克（mg）
- SheddingRate：脱落速率，单位为每小时的DNA拷贝数