RNA-seq analysis of poly-A-isolated RNA in Epcam-sorted KPC tumor cells from d21 tumors resected from Pf4-Cre and Dicer1fl/fl/Pf4-Cre mice.

Shown are the summary of total RNAs, mRNAs ranked by fold change, raw read counts, normalized read counts, rlog conversions, sample list, mRNAs by Ensembl ID, and predicted platelet miRNAs targeting the significantly increased mRNAs. Shown in the mRNAs ranged by fold change page (KO vs. WT) are Ensembl ID (A), gene symbol (B), Biotype (C) Chromosome (D), Gene description (E), Human homolog gene symbol (F), base Mean (G), log(2) fold change for KO (Dicer1fl/fl/Pf4-Cre) relative to WT (Pf4-Cre) (H), lfcSE (I), stat(J), raw p-value (K), and the log adjusted binomial p-value ranking statistic (L). Genes selected for protein expression analysis (Fig 4) are highlighted in yellow in the KO vs. WT table. ‘Dicer1 from Rowley et al’ shows the results of miRNet search for predicted miRNAs targeting the 548 significantly increased mRNAs in tumor cells (symbol, embl, entrez) in this study, then filtered for the top 44 miRNAs depleted in platelets from Dicer1fl/fl/Pf4-Cre mice compared to controls [52]. Search criteria were the list of mRNA embl IDs, and the selected species was Mus musculus. ‘miR from Sunderland’ shows the results of miRNet search for predicted miRNAs, filtered for the top 50 miRNAs enriched in human platelets as compiled in [55], targeting the 548 significantly increased mRNAs in tumor cells (symbol, embl, entrez) in this study. Search criteria were the list of mRNA embl IDs, and the selected species was Mus musculus. (XLSX)