Expression data from V194D Matn3 mutant Xbp1 WT and KO P5 mouse costochondral cell preparation

Multiple epiphyseal dysplasia (MED) results from mutations in cartilage structural proteins COMP, MATN3 and type IX collage. V194D Matn3 is a mouse model of MED in which a signifficant contribution of ER stress has been detected. In order to test whether ER stress contribution was a beneficial or detrimental component of the disease, V194D Matn3 mice were crossed with a cartilage specific knock-out of Xbp1, and transcriptomic analysis was performed using the Affymetrix 420A Mouse Array. Chondrocytes were extracted from postnatal day 5 ribs of Xbp1 WT and KO mice on V194D Matn3 or WT Matn3 background. RNA was extracted from isolated chondrocytes using Trizol reagentand pooled from 3 separate extractions. A GeneChip 3’ expression assay (Mouse430_2 Affymetrix) was used to analyse gene expression. Quality control checks for control hybridizations were performed using Microarray Suite 5.