Mechanomics RT-DC Set 04 - MCF10A-PIK3CA

Entry is a part of <b>Mechanomics</b> collection (10.6084/m9.figshare.c.5399826) that contains materials supporting findings presented in the following manuscript:<i>Urbanska, M., Ge, Y.</i> et al.,<br><b><i>De novo identification of universal cell mechanics gene signatures</i></b>BiorXiv: https://doi.org/10.1101/2021.04.26.441418<i>;</i><i> </i><i>first published:</i><i> </i>27th April 2021<i>, last updated:</i><i> </i>4th Nov 2024<br>eLife: https://doi.org/10.7554/eLife.87930<i>; first published:</i><i> </i>23rd March 2023<i>, last updated:</i><i> </i>27th Dec 2024<br>This dataset contains mechanical measurement of MCF10A cell lines with single-allele PIK3CA mutation E545K in exon 9 (ex9) or H1024R in exon 20 (ex20) (Juvin <i>et al.</i>, 2013, <i>PLoS One</i>). These cell lines were previously generated by homologous recombination by Horizon Discovery LTD, UK and were kindly provided, together with an isogenic wild type (wt) control, by L.R. Stephens.Lines:<br>• MCF10A wild type (wt)• MCF10A PIK3CA mutation E545K in exon 9 (ex9)*<br>• MCF10A H1024R in exon 20 (ex20).<br>*not used in the final version of the manuscript Measurements were performed using real-time deformability cytometry (RT-DC).<br><b>_rtdc.zip</b> - contains data acquired during the measurments in '*.rtdc' format<b>_tsv.zip</b> - contains extended data in a '*.tsv' format<br><b>_glmm.zip</b> - contains result summaries of statistical analysis comparing Young's moduli of the different cell types using generalised linear mixed effects models<br>Juvin, V. <i>et al.</i> Signaling via Class IA Phosphoinositide 3-Kinases (PI3K) in Human, Breast-Derived Cell Lines. <i>PLoS One</i><b> </b><b>8</b>, (2013). https://doi.org/10.1371/journal.pone.0075045<br>