YTHDF3 interacting mRNAs by RNA immunoprecipitation (RIP)-sequencing in human abdominal aortic aneurysm tissue

We performed a global screening for YTHDF3-binding mRNAs in an infrarenal abdominal aortic aneurysm patient (maximum aortic diameter=64.3 mm) sample, who have no comorbidity i.e. cancer, drug history, infection, or any other immune-related disease that might have influenced the study. After screening for reads in AAA tissue compare with anti-YTHDF3 group relative to IgG as control. The clustered libraries were loaded onto a reagent cartridge and forwarded for sequencing runs on an illumina Hiseq 4000.