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Revealing Missing Human Protein Isoforms Based on Ab Initio Prediction, RNA-seq and Proteomics

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https://figshare.com/articles/dataset/Revealing_Missing_Human_Protein_Isoforms_Based_on_Ab_Initio_Prediction_RNA_seq_and_Proteomics/1402048/13
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In this project, we design a methods to detect novel transcripts integrating ab initio prediction on genomic sequences and filtration with various RNA-seq data. 100 splice sites were selected and RT-PCR on 48 human tissues and cell lines (including a blank negative control) were conducted. The product of the same tissue or cell line were mixed and sequenced with Illumina Mi-seq.
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figshare
创建时间:
2016-01-19
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