Whole-transcriptome analysis of hematopoietic progenitors in the cranial dura, femur, and skull

Live Lin−/lowc-Kit+ singlets from the cranial dura, femur, and skull of adult mice were sorted for whole-transcriptome analysis. Leukocytes were mechanically isolated from the cranial dura, femur, and skull of ten 8 ~ 12-week-old C57BL/6 mice after intracardiac perfusion. Cells from the same compartment were mixed together and stained with DAPI and fluorescence-conjugated antibodies against c-Kit and lineage markers. Then DAPI−Lin−/lowc-Kit+ singlets were sorted and subjected to 10x Genomics single-cell RNA sequencing (scRNA-seq).