Mapping the downy mildew resistance locus RPF3 in spinach cv. Whale

This study aimed to finely map the downy mildew resistance locus <em>RPF3</em> in spinach, identify single nucleotide polymorphism (SNP) markers associated with the resistance, refine the candidate genes responsible for the resistance, and evaluate the prediction performance using multiple machine learning genomic prediction (GP) methods. The <em>RPF3</em> resistance regions were mapped to the 1.25 Mb and 2.73-2.74 Mb of Monoe-Viroflay chromosomes 3 based on the significant and consistent association of 20 SNP markers across three GWAS programs and ten models.