Bulk RNA sequencing of hippocampal neurons from TMSB4X conditional knockout (CKO) mice following mTBI

We generated a neuron-specific tdTomato red fluorescent protein-expressing Tmsb4x-CKO mouse model, Thy-1Cre/+ROSA26tdTomato/+ Tmsb4xf/y mice. This model specifically knocks out Tmsb4x in neurons while expressing the tdTomato fluorescent protein. We subsequently performed FACS to sort hippocampal neurons and extracted tdTomato+ cells for bulk RNA sequencing. The sequencing results revealed specific gene expression changes in the hippocampal neurons of CKO mice following mTBI. Overall design: RNA-seq profiles of the hippocampal neurons from Tmsb4x-CKO+mTBI mice(n=3), WT+mTBI mice(n=3), WT+Sham mic(n=3)e were generated.