Renal tubular Pck1 knockout mice showed albuminuria.

We aim to investigate the role of renal Pck1 on albuminuria. To further development of our gene expression approach to biodosimetry, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish between wild type mice. To elucidate the molecular mechanisms underlying the increase in albuminuria by our Pck-CKO (Pck1 conditional knockout) mice, we performed DNA microarray analysis using whole kidneys to examine the differences in gene expression between Pck1-CKO and wild-type control mice. Mice in the Pck1-CKO and WT(wild-type control) groups were killed at 32weeks of age. We obtained the whole kidneys to be used for total RNA isolation and subjected to analysis on microarray