Asymmetrical changes in chromatin and small RNAs contribute to altered gene expression and endosperm development in resynthesized wheat allotetraploids

We performed genome-wide analyses of mRNA and small RNA transcriptomes in the endosperm of two synthetic wheats SSAA and AADD that differ in seed fertility and size. Transcriptomes between the endosperm and root of AADD were also analyzed to test a developmental role. Immunostaining experiments were performed to analyze the changes of histone H3K9me2 modifications in the chromosomes of two wheat allotetraploids and their progenitors.