Relative amounts of mcm5s2U analyzed by HPLC in various elp3 mutants.
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Pseudouridine (Ψ) was used as an internal standard. The numbers given are the ratios of mcm5s2U to Ψ in total tRNA isolated from various mutants normalized to the ratio in the wild type SUP4 strain. Values represent the average of three independent experiments, except for elp3-Y540A SUP4 and elp3-Y541A SUP4 that are repeated five times. Standard deviation is shown. ND indicates ‘not detected’. Abbreviations: (Ψ) pseudouridine; and (mcm5s2U) 5-methoxycarbonylmethyl-uridine.
创建时间：
2015-12-02
