Exosomal miRNA signatures of amniotic fluid in mice

To investigate the exosomal miRNA changes under the inflammatory reaction, LPS (100 μg/kg body weight) was intraperitoneally injected into the mice at 15 days of pregnancy. Premature births has been found after approximately 48 h of treatment. When bleeding found in vagina, the uterus and other embryo without breaking water were selected in asepsis condition. The amniotic fluid were selected and isolated exosome to analyze the expression of miRNAs compared with cesarean sections. The exosomes derived from LPS-treated mice were tested the miRNAs expression, the exosomes derived from cesarean sections as a control.