Transcription profiling by high throughput sequencing of mouse wild type and Stat5-mutant mammary tissues at parturition and early pregnancy

To dissect regulatory processes of cell proliferation and differentiation we generated mouse strains carrying any combination of the four Stat5 alleles, thus expressing STAT5 from 0 to 100%. RNA-Seq analyses revealed that different STAT5 levels activate specific genetic programs linked to cell proliferation and differentiation. We refer to wild-type mice and Stat5abfl/fl mice as AABB mice; Stat5abfl/fl;MMTV-Cre (with Stat5ab-deficient mammary epithelial cells) as Null mice; Stat5a-/- mice as BB mice; Stat5b-/- mice as AA mice; Stat5ab+/null mice as AB mice.为解析细胞增殖与分化的调控机制，我们构建了携带四种信号转导与转录激活因子5（Stat5）等位基因任意组合的小鼠品系，由此可使STAT5的表达量覆盖0至100%的区间。通过RNA测序（RNA-Seq）分析发现，不同水平的STAT5可激活与细胞增殖、分化相关的特异性遗传程序。我们将野生型小鼠与Stat5abfl/fl小鼠命名为AABB小鼠；将携带Stat5ab缺陷乳腺上皮细胞的Stat5abfl/fl;MMTV-Cre小鼠命名为Null小鼠；将Stat5a-/-小鼠命名为BB小鼠；将Stat5b-/-小鼠命名为AA小鼠；将Stat5ab+/null小鼠命名为AB小鼠。