The detection of Prunus virus F and its distribution in Hungarian sour cherry orchards

Viromes of sour and sweet cherry trees in Hungarian germ line collections were surveyed using small RNA HTS as an unbiased method. RNA from leaf samples of different cultivars were purified and used to produce seven pools from which small RNA HTS libraries were prepared. The sequenced reads were analyzed using bioinformatic methods to revel the presence of viruses in the samples. Presence of the viruses were validated using RT-PCR. sRNA HTS based diagnostics from leaf samples of the presenting viruses in different sour and sweet cherry cultivars