Role of RBM14 in double-strand break repair.

We demonstrated RBM14 is required for the canonical non-homologous recombination (cNHEJ) pathway. To understand roles of RBM14 in cNHEJ, we performed ChIP-seq and DRIP-seq analyses to detect the binding of RBM14 at double-strand break (DSB) sites which were induced by I-PpoI endonuclease. Furthermore, DRIP-seq analyses were performed to detect RNA-DNA hybrids at the DSB sites. RNA-seq analyses were performed to detect on-going transcription in the I-PpoI-induced DSB system.