Epicoccum nigrum P16 Genome sequencing and assembly

In recent years, the interest in endophytic fungi as a prolific source of new species and also because of its potential uses in biological control and production of secondary metabolites with different biological activities has increased significantly. Despite the increasing discovery of new compounds from endophytic fungi, there is a huge gap in knowledge of biosynthetic pathways for the production of bioactive metabolites. The vast majority of endophytes that produce new compounds have no efficient systems for genetic manipulation. Since the use of genome mining approaches has led to the discovery of new molecules in different microorganisms, the application of this technology to endophytic fungi allowing the expanding the metabolic potential of these organisms. Previous studies have shown that the fungus Epicoccum nigrum is commonly isolated as endophytic in sugarcane and an extensive genetic variation can be found among isolates from a single location and host. This fungus is known for its uses as biological control agent and production of a range of secondary metabolites with high biotechnological value. In this context, this project proposes to identify potential genes and metabolic pathways related to the biosynthesis of antimicrobial metabolites from Epicoccum nigrum and determine the identity of the compounds. Additionally, this project aim to sequence the genome of E. nigrum and compare identified genes and metabolic pathways with the pathways and genes associated to bioactive compounds.