Hybrid sequencing of persistent carriage S. aureus isolate MHO_001

The Oxford Nanopore MinION is a mobile DNA sequencer that can produce long read sequences with a short turn-around time. Here we report the first demonstration of full genome closure using Oxford Nanopore native barcoding when applied to a multiplexed library of 12 samples and combined with existing Illumina short-read data. This paves the way for the closure of multiple bacterial genomes from a single MinION sequencing run, given the availability of existing short-read data. The strain we used, MHO_001, represents the important community-acquired methicillin resistant Staphylococcus aureus lineage USA300.