Data from: SNP markers tightly linked to root knot nematode resistance in grapevine (Vitis cinerea) identified by a genotyping-by-sequencing approach followed by Sequenom MassARRAY validation

Plant parasitic nematodes, including root knot nematode Meloidogyne
species, cause extensive damage to agriculture and horticultural crops. As
Vitis vinifera cultivars are susceptible to root knot nematode parasitism,
rootstocks resistant to these soils pests provide a sustainable approach
to maintain grapevine production. Currently, most of the commercially
available root knot nematode resistant rootstocks are highly vigorous and
take up excess potassium, which reduces wine quality. As a result, there
is a pressing need to breed new root knot nematode resistant rootstocks,
which have no impact on wine quality. To develop molecular markers that
predict root knot nematode resistance for marker assisted breeding, a
genetic approach was employed to identify a root knot nematode resistance
locus in grapevine. To this end, a Meloidogyne javanica resistant Vitis
cinerea accession was crossed to a susceptible Vitis vinifera cultivar
Riesling and results from screening the F1 individuals support a model
that root knot nematode resistance, is conferred by a single dominant
allele, referred as MELOIDOGYNE JAVANICA RESISTANCE1 (MJR1). Further, MJR1
resistance appears to be mediated by a hypersensitive response that occurs
in the root apical meristem. Single nucleotide polymorphisms (SNPs) were
identified using genotyping-by-sequencing and results from association and
genetic mapping identified the MJR1 locus, which is located on chromosome
18 in the Vitis cinerea accession. Validation of the SNPs linked to the
MJR1 locus using a Sequenom MassARRAY platform found that only 50% could
be validated. The validated SNPs that flank and co-segregate with the MJR1
locus can be used for marker-assisted selection for Meloidogyne javanica
resistance in grapevine.