Sleuth Object (S.avenae head/body)

Differential gene expression analysis where RNA sequencing reads from the Sitobion avenae were pseudoaligned to the reference transcriptome with Kallisto1. Samples from heads were compared to samples from body using Sleuth2. The sleuth object (generated with version 0.30.0) can be used with sleuth_live() to examine the expression at a transcript level.Details on installation of Sleuth can be found at https://github.com/pachterlab/sleuth.Once Sleuth is installed in R then simply read in the sleuth object and run sleuth live as follows:library(sleuth)sleuthObject sleuth_live(sleuthObject) 1Bray, N.L., Pimentel, H., Melsted, P. and Pachter, L., 2016. Near-optimal probabilistic RNA-seq quantification. Nature biotechnology, 34(5), pp.525-527.2Pimentel, H., Bray, N.L., Puente, S., Melsted, P. and Pachter, L., 2017. Differential analysis of RNA-seq incorporating quantification uncertainty. Nature methods, 14(7), p.687.