ARID1A regulates RNAPII pausing in ovarian clear cell carcinoma

Immunoblots were made to check knock-down levels for ARID1A and ARID1B, overexpression and time-course regulation for ARID1A and ARID1B, and protein levels for pausing factors as RNAPII, NELF and SPT5.本研究通过免疫印迹实验（Immunoblots）检测了ARID1A与ARID1B的基因敲低水平、ARID1A和ARID1B的过表达情况及时序调控规律，同时检测了转录暂停因子RNA聚合酶II（RNAPII）、NELF与SPT5的蛋白水平。