Transcriptomic analysis by AmpliSeq Ion Torrent to study the involvement of CXXC5 in the regulation of TGFβ/BMP target genes in glioblastoma. Transcriptomic analysis by AmpliSeq Ion Torrent to study the involvement of CXXC5 in the regulation of TGFβ/BMP target genes in glioblastoma

The aim of this work was to elucidate the role of the transcription factor CXXC5 in glioblastoma and study its involvement in the regulation of TGFβ and BMP target genes in this context. To this end we used the patient derived glioblastoma stem cells U3034MG that were either transfected with siRNA targeting CXXC5 or with a non-targeting pool (as control) followed by stimulation with either 5ng/ml TGFβ or 30ng/ml BMP7 for 24 h. Then we analysed how silencing CXXC5 affects the expression of TGFβ and/or BMP7 target genes using Ion torrent AmpliseqTM.