High-throughput sequencing of small RNAs in chicken somite embryos
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High-throughput sequencing of small RNAs in chicken somite embryos Overall design: Dissected tissue white leghorn chicken embryos was disaggregated and RNA extracted using the miRVana kit (Ambion). Small RNA fraction between 19 and 24 nt was isolated from 15% denaturing polyacrylamide gel and 15 micro gram was ligated to Solexa adaptors (Illumina) without de-phosphorylating and re-phosphorylating. The short RNAs were converted to DNA by RT-PCR following the Illumina protocol.
创建时间：
2019-09-23
