Continuous data of Lipoxygenase activity in potato cultivars

This study aimed to determine the lipoxygenase (LOX) activity in the fruit juice of five industrial potato cultivars and to assess whether significant differences exist among them. The dataset is organized into three sheets:

“Standard curve” sheet:
This sheet contains five data points ranging from 10 to 100 µM 13-hydroperoxyoctadecadienoic acid (13-HPODE) along with their corresponding absorbance values at 234 nm. The data demonstrate a linear relationship between 13-HPODE concentration and absorbance at 234 nm.

“Raw data” sheet:
This sheet includes the primary measurements of LOX activity for the five potato cultivars. Each dataset consists of a blank sample (sample + buffer, without substrate) and three biological replicates (R1, R2, and R3), each measured in technical triplicates. Absorbance was recorded every 15 seconds over a 5-minute period. Data processing was carried out as follows:

1) (Highlighted in yellow) Technical replicates were averaged for each biological replicate, and the blank value was subtracted.
2) (Highlighted in yellow) Absorbance values were converted to 13-HPODE equivalents using the standard curve from the “Standard curve” sheet.
3) (Highlighted in yellow) LOX activity (expressed as µM/min from step 2) was normalized to mM 13-HPODE/min per mg protein using the measured protein content of each juice sample.

“Final result” sheet:
This sheet summarizes the calculated LOX activity for each biological replicate (Rate R1, Rate R2, and Rate R3), along with the mean ± standard deviation (STD). The results indicate clear differences in LOX activity among the potato cultivars.

Further details on data acquisition are provided in the “Steps to reproduce” section.

Overall, the data demonstrate that potato cultivars differ significantly in lipoxygenase activity, a key factor influencing lipid oxidation.