DataSheet_1_Dynamics of the Apostichopus californicus-associated flavivirus under suboxic conditions and organic matter amendment.pdf

Flaviviruses cause some of the most detrimental vertebrate diseases, yet little is known of their impacts on invertebrates. Microbial activities at the animal-water interface are hypothesized to influence viral replication and possibly contribute to pathology of echinoderm wasting diseases due to hypoxic stress. We assessed the impacts of enhanced microbial production and suboxic stress on Apostichopus californicus associated flavivirus (PcaFV) load in a mesocosm experiment. Organic matter amendment and suboxic stress resulted in lower PcaFV load, which also correlated negatively with animal mass loss and microbial activity at the animal-water interface. These data suggest that PcaFV replication and persistence was best supported in healthier specimens. Our results do not support the hypothesis that suboxic stress or microbial activity promote PcaFV replication, but rather that PcaFV appears to be a neutral or beneficial symbiont of Apostichopus californicus.

黄病毒引起了一些最具破坏性的脊椎动物疾病，然而，关于其对无脊椎动物的影响知之甚少。动物-水界面处的微生物活动被假设会影响病毒的复制，并可能因缺氧应激而加剧棘皮动物消瘦疾病的发生。我们在一个中尺度生态系统中评估了增强的微生物生产和亚缺氧应激对加州海胆相关黄病毒（PcaFV）载量的影响。有机物质改良和亚缺氧应激导致PcaFV载量降低，这与动物体重减轻和动物-水界面处的微生物活动呈负相关。这些数据表明，PcaFV的复制和持续存在在健康的样本中得到了最佳支持。我们的结果不支持亚缺氧应激或微生物活动促进PcaFV复制的假设，反而表明PcaFV似乎是一种对加州海胆中性的或有益的共生体。