Architecture mechanics mediated osteogenic progression in bone regeneration of artificial scaffolds

Scaffold architecture exerts a considerable influence on the osteogenic effect through stress transmission, as the deformation of scaffolds alters the mechanical microenvironment of cells adhering to scaffold surface. Despite extensive researches on bone regeneration influenced by scaffold architecture, present studies have not addressed the biological mechanism underlying scaffold architecture-induced stress stimulation (SASS) on cells yet, posing a great challenge in revealing the biomechanical cues between scaffold architecture and osteogenic progression. Therefore, Graphite (GP), Fullerene (FL), and Diamond (DM) scaffolds with gradient stress-stimulation to cells after deformation were prepared. Moreover, we analyzed the results of the finite element simulation of the compression and recovery process of the three types of scaffolds, and tested the macroscopic mechanical and viscoelastic properties. The cellular biomechanical mechanisms of SASS through single-cell RNA sequencing indi..., , # Architecture mechanics mediated osteogenic progression in bone regeneration of artificial scaffolds

Dataset DOI: [10.5061/dryad.dr7sqvb8w](10.5061/dryad.dr7sqvb8w)

## Description of the data and file structure

We summarized the finite element simulation of the micro-strain distribution, macro-mechanical, and viscoelastic properties of GP, FL, and DM scaffolds during the compression and recovery process. One month after implantation in the femur of rats, the new bone at the defect was removed, and single-cell sequencing was performed. The KEGG, GO enrichment, and gene expression differences of mesenchymal stem cells (MSCs), osteoclasts (OCs), and osteoblasts (OBs) cell clusters in the three scaffolds were analyzed. In the *in vivo* experiments, the parameters of new bone, semi-quantitative analysis of IHC and IF were analyzed.

### Files and variables

#### File: Data\_files.zip

**Description:** 

**Porosity&surface-volume ratio.xlsx**: Porosity and surface-volume ratio of GP, FL, ...,