Analysis of miRNA sublocalization in cisplatin-resistant versus parental CAL-27 cells

Chemoresistance frequently leads to therapeutic failure in oral squamous cell carcinoma (OSCC). While miRNA have been widely reported having global regulatory roles in cancer biological properties, yet their localiztion alteration under cisplatin treatment has not been clarified. In this study, to identify the sublocalization characteristics of miRNAs induced by chemotherapy, especially the specific mitochondrial localization of miRNA, we profile the expression of miRNAs in cisplatin-resistant and parental TSCC cells using miRNA microarrays. We investigated the differential localization of miRNA in stable cisplatin-resistant CAL-27 cells and parental CAL-27 cells. Sample labeling and array hybridization were performed by Agilent human miRNA (8*60K) V19.0 microarrays.