RNA-Seq analysis of the spongy tissue disorder in Mangifera indica Cv. Alphonso.

Transcriptome analysis of the spongy tissue disorder in Mangifera indica Cv. Alphonso was carried out individually at two stages of ripening (Table green and mid ripe) in the healthy and the spongy mesocarp. Sequencing was carried out using Illumina technique. Total number of raw reads generated from each stage of tissue ranged from 5-7 Gb. Importantly, 30,582 transcripts were annotated and 1,218 enzyme commission number were assigned from oxidoreductase, transferase, hydrolase, ligase, lyase and isomerase classes. Various differential transcripts (p < 0.05) were highlighted within the healthy and the spongy tissue during ripening. These were involved in biological pathways related to metabolism of fatty acids, sugars, amino acids etc. The altered expression pattern of these transcripts indicated the change in primary as well as secondary metabolism of fruit under the spongy tissue condition. Interestingly, several stress related transcripts were differentially expressed in both the stages of the spongy mesocarp which has advocated the role of abiotic stress in the spongy tissue disorder. Transcriptome analysis of two ripening stages (table green (5DAH) and mid ripe (10DAH)) of Alphonso mango under the healthy and the spongy tissue disorder condition.