Detection limit of hantavirus RT-qPCR and Semi-nested RT-PCR assays.

NIAID Data Ecosystem2026-03-11 收录

数据链接：

https://figshare.com/articles/dataset/Detection_limit_of_hantavirus_RT-qPCR_and_Semi-nested_RT-PCR_assays_/11462727 数据链接链接失效反馈

官方服务：

资源简介：

Detection limit of hantavirus RT-qPCR and Semi-nested RT-PCR assays.

创建时间：

2019-12-26

Repository of raw qPCR data to assess Sin Nombre hantavirus presence in lung tissues and replication in vitro

汉坦病毒检测

RNA定量

Background: Orthohantaviruses are negative-sense RNA viruses that can cause hantavirus cardiopulmonary syndrome (HCPS) in humans. In the United States, Sin Nombre orthohantavirus (SNV) is the primary

DataONE2025-02-04 更新80

The result of in-silico NCBI Primer-BLAST, indicating the number of matches and number of mismatches with the intended target and non-target hits, along with sensitivity of the specific primers both individually and when used in the mix estimated by Spectra Max Gemini XPS microplate reader.

引物特异性分析

检测灵敏度

NIAID Data Ecosystem50

Determining sensitivity of the Target Selector Assay at or near single copy input levels.

检测灵敏度

低拷贝数突变检测

Column ‘ddPCR Copies (Reference)’ shows ddPCR validation of controls for cell lines containing mutations of interest. Source DNA was isolated from named cell lines or commercially sourced human placen

Figshare2019-10-03 更新30

Detection limit of the MIP assay.

检测灵敏度

基因组DNA定量

The MIP (MIPFOC01) was mixed with different amounts of Foc genomic DNA (160 ng, 80 ng, 40 ng, 20 ng, 10 ng, 5 ng, 2.5 ng and 1.25 ng) in triplicates and analyzed in a MIP assay. The PCR amplicon was e

Figshare2016-02-23 更新50

Analytical conditions in the separation and quantification of glucuronides.

葡萄糖醛酸苷分析

检测灵敏度

1LOD, limit of detection; LOQ, limit of quantification; values are calculated assuming maximal injection volume;2The UV signal for was correlated with fluoresence for enhanced sensitivity.

Figshare2015-12-02 更新40

Detection limit of hantavirus RT-qPCR and Semi-nested RT-PCR assays.

资源简介：

相关数据集