Rhizosphere Soil of Tibet Barley Raw sequence reads

Continuous cropping regulates community structure and function of rhizosphere soil microbes; however, the the effect of continuous cropping on the community structure and function of rhizosphere fungal community of plants cultivated at high-altitude regions are less understood. In this study, 18S rRNA gene high-throughput sequencing was applied to examine the rhizosphere fungal community structure during continuous cropping of Tibet barley (a principal cereal cultivated on Qinghai-Tibetan Plateau). The results showed that Chao1 and phylogenetic diversity (PD) indice were declined along with the increased cropping years. The relative abundance of genus Cystofilobasidium, Mucor, and Ustilago were successively increased, whereas the abundance of Fusarium exibited decreasing pattern during continuous cropping. Futhermore, ecological groups were inferred with FUNGuild, that saprotrophs, pathogens, and symbiotroph were the domiantedly groups in rhizosphere soil, and these three trophic modes all increased significantly with the continuous cropping. During continuous cropping, plant-pathogen fungal genus Parastagonospora and Ustilago were remarkablely increased. Additionally, the endophyte fungi Verticillium dramatically increased with the duration of continuous cropping. Collectively, continuous cropping of Tibet barley increased potential plant-pathogen and endophyte fungi in rhizosphere soil. Thus, the developement of sustainable farming practices to reduce abundance of harmful fungi is vital for Tibet barley growth and production during continuous cropping of plants cultivated at high-altitude regions.