Whole genome sequence data of Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway- Part 2

This dataset contains raw whole-genome sequencing data (FASTQ files) for wild-type and riboflavin biosynthetic gene deletion mutants of Mycobacterium tuberculosis and Mycolicibacterium smegmatis. These strains were generated to investigate the source of metabolic antigens recognized by mucosal-associated invariant T (MAIT) cells, which detect intermediates from the riboflavin biosynthetic pathway. To confirm successful gene deletions and rule out off-target effects, whole-genome sequencing was performed for each mutant and its corresponding wild-type strain. This dataset supports the verification of targeted genetic modifications and enables comparative genomic analyses related to MR1 antigen production in mycobacteria., , # Whole genome sequence data of Mycobacterium tuberculosis and Mycolicibacterium smegmatis mutants of the riboflavin biosynthetic pathway- Part 2

Dataset DOI: [10.5061/dryad.vq83bk454](10.5061/dryad.vq83bk454)

## Description of the data and file structure

Whole Genome Sequence of wild type strain and riboflavin biosynthetic pathway mutants of *Mycobacterium tuberculosis* (Mtb) and *Mycolicibacterium smegmatis* (Msm). Also, this dataset has part 1 which is available at https://doi.org/10.5061/dryad.4tmpg4fnv

Naming convention for files: 

For file names starting in digits

1. Strain number (1: Wild Type, 2: *ΔfbiC, *3: ΔribH2*, 4: *ΔribH2 ΔribH1,* 5: ΔribH2::ribH2, *6: *ΔfbiC::fbiC, 7: ΔribH2 ΔribH:: ribH2 ribH1*) 2. Sample Number 3. Read (Forward/Reverse) 6. Replicate

e.g. For file name \"7_S23_L004_R2_001\"Strain number: 7 (*ΔribH2 ΔribH:: ribH2 ribH1*); Sample Number: S23; Read: R2 (Reverse Read); Replicate: 001

OR

For file names starting in \"Dobos\"

1. Lab name 2. Strain Name 3....,