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Transcriptome Analysis of the Human Cytomegalovirus Using Pacific Biosciences RSII Platform. HCMV transcriptome profiling by PacBio sequencing

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NIAID Data Ecosystem2026-03-10 收录
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To assess the transcriptional capacity of the Human cytomegalovirus (HCMV), total RNA was isolated from from HCMV infected MRC-5 (human lung fibroblast) cells and reverse transcribed using SuperScript IV using oligo(dT) primers for the poly(A)-selected fraction and random primers for the rRNA-depleted fraction of the RNAs. cDNAs were sequenced using the Pacific Biosciences RSII platform.

创建时间:
2017-09-05
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