Fluorescence micrographs demonstrating the distribution of inherently fluorescent Janus kinase 3 inhibitors

The dataset contains supplementary images for the publication "Inherent fluorescence demonstrates immunotropic properties for novel Janus kinase 3 inhibitors". In short, cells were incubated with fluorescent JAK3 inhibitors or control fluorophore 13 (appearing in green) as well as stains for lysosomes (appearing in red) or nuclei (appearing in blue). For in vivo samples (tail blood and organs), C57/BL6 mice were treated p.o. with the aforementioned JAK3 inhibitors or the control. Again, nuclei were stained blue and compounds appear in green. File names indicate the test compound used (1-13), the sample type (cell type or organ), incubation time (for in vitro samples), sampling time post treatment (for in vivo tail blood samples) and the level of magnification. "Overlay" indicates a merge of channels for compound fluorescence, lysosome and nucleus stains. "Overview" indicates a merge of singular captures to give an overview of the entire organ sample. HBC = human buffy coat HMC3 = human microglia cells PBMCs = peripheral blood mononucleated cells本数据集为论文《固有荧光特性验证新型Janus激酶3（Janus kinase 3, JAK3）抑制剂的免疫趋向性》的补充图像资料。简言之，实验中将细胞与荧光标记的JAK3抑制剂或对照荧光团13（呈绿色）共同孵育，同时使用溶酶体染色剂（呈红色）或细胞核染色剂（呈蓝色）进行标记。针对体内样本（尾血及脏器样本），我们对C57/BL6小鼠经口（p.o.）给予前述JAK3抑制剂或对照试剂。此时细胞核仍被染为蓝色，受试化合物呈绿色荧光。文件名包含以下信息：所用受试化合物（编号1-13）、样本类型（细胞系或脏器）、孵育时长（体外样本）、给药后采样时长（体内尾血样本）以及放大倍率。“Overlay”代表化合物荧光、溶酶体染色与细胞核染色的通道叠加图像。“Overview”代表单张拍摄图像的拼接图，用于展示完整脏器样本的全貌。HBC = 人类血沉棕黄层（human buffy coat, HBC）；HMC3 = 人类小胶质细胞（human microglia cells, HMC3）；PBMCs = 外周血单个核细胞（peripheral blood mononucleated cells, PBMCs）