Elevated levels of T-lymphocytes within MCMV-infected brain following acute Treg depletion.

(A) Schematic representation of diphtheria toxin injection given to Foxp3-DTR transgenic mice during acute phase of viral brain infection to deplete Treg cells. (B) Absolute numbers of Treg cells were calculated based on flow cytometric analysis from MCMV infected brain with and without DTx treatment at 7, 14, and 30 dpi. (C) Absolute numbers of CD8+ cells were determined within Dtx-treated and untreated, MCMV-infected brains at indicated the time points. (D) Absolute numbers of CD4+cells observed at the indicated time points. Pooled data are presented as mean ±SD of two experiments using six animals per treatment group/time point. *p < 0.01 and **p < 0.001 DTx- versus DTx+ MCMV-infected