Single-nucleus sequencing data from developing and adult mouse olivocochlear neurons

Transcriptional diversity of mouse olivocochlear neurons (OCNs) was examined using single-nucleus sequencing of brainstem cholinergic neurons at P1, P5, and P26-P28. This dataset includes multiple brainstem cell types, including OCNs and several other populations of cranial motor neurons. Cholinergic nuclei were labeled using ChAT-Cre to drive expression of the nuclear localized Rosa26-Sun1-GFP reporter. GFP+ cells from the ventral brainstem were selected via fluorescence-activated cell sorting (FACS) and nuclei were captured using the 10x genomics single-cell 3' platform.本研究通过对出生后第1天（P1）、第5天（P5）及第26至28天（P26-P28）的脑干胆碱能神经元进行单细胞核测序，解析了小鼠橄榄耳蜗神经元（olivocochlear neurons, OCNs）的转录多样性。该数据集涵盖多种脑干细胞类型，不仅包含橄榄耳蜗神经元，还包括数群其他颅运动神经元。研究通过ChAT-Cre驱动核定位型Rosa26-Sun1-GFP报告基因的表达，对胆碱能细胞核进行标记；随后通过荧光激活细胞分选术（fluorescence-activated cell sorting, FACS）分选得到脑干腹侧的绿色荧光蛋白阳性（GFP+）细胞，并采用10x Genomics单细胞3'端测序平台完成细胞核捕获。