BestKeeper results obtained for bone marrow-derived mesenchymal stromal cells.

The last column (BK, n = 3) shows the parameters obtained after combining all stably expressed genes (B2M, RPL13A and HPRT1), so they can be use as a single normalization factor. n: number of samples; BK: BestKeeper; GM [Cq]: geometric mean of Cq; AM [Cq]: arithmetic mean of Cq; Min [Cq]: minimum value of Cq; Max [Cq]: maximum value of Cq; SD [± Cq]: standard deviation of the Cq; CV [% Cq]: coefficient of variation expressed as a percentage on the Cq level; Min [x-fold]: minimum value of expression levels expressed as an absolute x-fold; Max [x-fold]: maximum value of expression levels expressed as an absolute x-fold; SD [± x-fold]: standard deviation of the absolute regulation coefficients. SD values obtained for ACTB (1.42) and GAPDH (1.03) are higher than cutoff (1.0), so both genes were defined as unstably expressed genes and therefore were not considered for BK (n = 3) calculations.